Evaluation of antibiotic residues in the liver of broiler by four-plate method in Kazerun city
Evaluation of antibiotic residues in the liver of broiler by four-plate method in Kazerun city
Volume 2, Issue 5, 2018, Page 273 - 282
Authors : Gholamhossein Habibi

Abstract :
Drugs and antibiotics are widely used in birds for the treatment, prevention, and stimulation of growth. In addition to the desired therapeutic effects or even adverse side effects, the use of these drugs can indirectly affect public health.In this study, 100 packets of livers supplied to the market over a period of three months were collected from protein stores at the city level. First, the susceptible and standard strain of Escherichia coli prepared from the Pasteur Institute of Iran was cultivated and antibiogram was used to determine the sensitivity of the strain. Then, suspension 0.5 McFarland was prepared and Müller-Hinton media with acidity of 7.2 were prepared and autoclaved. After surface and uniform culturing of Müller-Hinton plates with cotton swab with Escherichia coli strains, one fragment of each liver was cut and transferred to surface of four media with sterilizing forceps and by keeping the appropriate distance from the edges. Then, it was placed at 37 ℃ for 24 hours. Finally, the samples were examined in terms of the presence or absence of antibiotics. Out of 100 samples examined, 69 samples with negative result (non-antibiotic) and 31 samples with positive result (containing antibiotic) were observed. The highest frequency was related to non-antibiotic samples. Since the drug residue disrupts public health, it is important to contribute to the health of the community by observing the withdrawal time before slaughter and forcing the producers to measure drug residue at the end of the breeding period.

Keywords :
Drug residue, Liver, Broiler, Four-plate method